N. Leigh Anderson Biography N. Leigh Anderson, Ph.D. is Founder and CEO of the Plasma Proteome Institute, Washington DC (www.plasmaproteome.org). The Institute aims to foster a comprehensive exploration of the proteins of human blood plasma (the plasma proteome) and the rapid application of novel protein measurements in clinical diagnostics. Prior to founding PPI, Dr. Anderson was Chief Scientific Officer at Large Scale Biology Corporation (Nasdaq: LSBC), whose proteomics division he founded in 1985 (as Large Scale Biology) prior to its merger in 1999 with Biosource Technologies. At LSBC, he developed the first automated two-dimensional electrophoresis technology platform for proteomics research, and pioneered a range of applications in drug discovery, toxicology and surrogate markers. Dr. Anderson obtained his B.A. in Physics with honors from Yale and a Ph.D. in Molecular Biology from Cambridge University (England) where he worked with M. F. Perutz as a Churchill Fellow at the MRC Laboratory of Molecular Biology. Subsequently he co-founded (with Dr. Norman Anderson) the Molecular Anatomy Program at the Argonne National Laboratory (Chicago) where his work in the development of 2-D electrophoresis and molecular database technology earned him, among other distinctions, the American Association for Clinical Chemistry's Young Investigator Award for 1982 and the 1983 Pittsburgh Analytical Chemistry Award. The Andersons together undertook the first systematic "proteomics" investigations of human plasma by 2-D electrophoresis, published in PNAS in 1977 (1), and further analyzed plasma protein microheterogeneity (2), and the properties of plasma antibodies (3). At LSBC, Leigh Anderson initiated a database of plasma proteins observed by 2-D electrophoresis (4), a collaboration with Pfizer that provided early direct evidence of the utility of multiple plasma protein marker panels in the study of inflammation and anti-inflammatory drug effects (5), a program to develop immunosubtraction processes for removal of the 10+ most abundant plasma proteins (6; subsequently licensed to Agilent) and the use of additional chromatographic fractionation processes for uncovering minor protein constitutents (7). Most recently, Dr. Anderson has reviewed the status of the plasma proteome (8), and initiated programs at PPI to develop a database of proteins in plasma (9) and a new method form measuring candidate protein markers in plasma (SISCAPA: 10,11). Dr. Anderson holds more than 30 patents and has written one book and over 130 scientific publications, mainly in the areas of proteomics and its applications. 1. Anderson, L. and Anderson, N.G. High resolution two-dimensional electrophoresis of human plasma proteins. Proc Natl Acad Sci U S A 74, 5421-5 (1977). 2. Anderson, N.L. and Anderson, N.G. Microheterogeneity of serum transferrin, haptoglobin and alpha 2 HS glycoprotein examined by high resolution two-dimensional electrophoresis. Biochem Biophys Res Commun 88, 258-65 (1979). 3. Anderson NL. High resolution two-dimensional electrophoretic mapping of immunoglobulin light chains. Immunol Lett 2, 195-199 (1981). 4. Anderson, N.L. and Anderson, N.G. A two-dimensional gel database of human plasma proteins. Electrophoresis 12, 883-906 (1991). 5. Doherty, N.S. et al. Analysis of changes in acute-phase plasma proteins in an acute inflammatory response and in rheumatoid arthritis using two-dimensional gel electrophoresis. Electrophoresis 19, 355-63 (1998). 6. Pieper, R., et al, The human serum proteome: Display of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins. Proteomics 3(7): 1345-64. (2003). 7. Pieper, R., et al, Multi-component immunoaffinity subtraction chromatography: An innovative step towards a comprehensive survey of the human plasma proteome. Proteomics 3(4): 422-32 (2003). 8. Anderson, N.L. and Anderson, N.G. The human plasma proteome: History, character, and diagnostic prospects. Molecular and Cellular Proteomics, 1.11, 845-867 (2002) 9. Anderson, N.L.,et al. The Human Plasma Proteome: A Non-Redundant List Developed by Combination of Four Separate Sources, Mol Cell Proteomics 3: 311-326 (2004). 10. Anderson, N.L.,et al. An effective and rapid method for functional characterization of immunoadsorbents using POROS® beads and flow cytometry. Journal of Proteome Research, in press (2004). 11. Anderson, N.L.,et al. Mass Spectrometric Quantitation of Peptides and Proteins Using Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA). Journal of Proteome Research, in press (2004). Bibliography